Coding
NAR

Part:BBa_K4354006:Design

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-22)


assimilatory nitrate reductase [Dunaliella tertiolecta]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 367
    Illegal PstI site found at 1523
    Illegal PstI site found at 1529
    Illegal PstI site found at 2287
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1503
    Illegal PstI site found at 367
    Illegal PstI site found at 1523
    Illegal PstI site found at 1529
    Illegal PstI site found at 2287
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1088
    Illegal BamHI site found at 203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 367
    Illegal PstI site found at 1523
    Illegal PstI site found at 1529
    Illegal PstI site found at 2287
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 367
    Illegal PstI site found at 1523
    Illegal PstI site found at 1529
    Illegal PstI site found at 2287
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 212
    Illegal SapI.rc site found at 355


Design Notes

By cloning AMO,HAO,NAR,NAR,NOR,NOS to the Bacillus subtilis, We want to make it an engineering strain that could remove the nitrogenous compounds in the waste water.


Source

Our team search its sequence from NCBI(AAL79356) and synthesize it.It coms from Dunaliella tertiolecta.

References

1.Song Qin, Xu Lei.Advancement of Enzymology of Heterotrophic Nitrification.BIOTECHNOLOGY BULLETIN.DOI:10.13560/j.cnki.biotech.bull.1985.2008.05.009 2.Xu Tao.METABOLIC CHARACTERISTICS OF HETEROTROPHIC NITRIFYING-AEROBIC DENITRIFYING STRAIN DIAPHOROBACTER SP.PD-7.Taiyuan University of Technology in China.2015 May10.